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Introduction EDC HCl, also known as 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, is a water-soluble carbodiimide reagent widely used in peptide synthesis, bioconjugation, and crosslinking reactions. Known as a zero-length crosslinker, EDC HCl facilitates the direct coupling of carboxylic acid groups with amines, forming stable amide bonds without introducing additional linker atoms. Its unique solubility and effectiveness in aqueous buffers have made it a preferred reagent in life sciences, pharmaceutical chemistry, and material sciences. Chemical Information IUPAC Name: 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride Molecular Formula: C8H17N3·HCl Molecular Weight: 191.7 g/mol CAS Number: 25952-53-8 Form: White to off-white crystalline powder Solubility: Soluble in water, DMSO, DMF EDC is often used in conjunction with NHS (N-hydroxysuccinimide) or Sulfo-NHS to improve coupling efficiency and reduce side reactions.

p-Tolunitrile, also known as 4-methylbenzonitrile, is an aromatic organic compound with the formula CH₃C₆H₄CN. It consists of a benzene ring substituted with a methyl group at the para (4th) position and a nitrile group (-CN). It appears as a colorless to pale yellow liquid or solid. p-Tolunitrile is widely used as an intermediate in the production of dyes, pharmaceuticals, and agrochemicals. It is important in organic synthesis, especially in forming amines and acids.

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